Human Thyroid peroxidase / TPO Protein (257 Ser/Ala, 725 Pro/Thr, His Tag)
MSA,TDH2A,TPX
- 100ug (NPP4310) Please inquiry
Catalog Number | P13194-H08B |
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Organism Species | Human |
Host | Baculovirus-Insect Cells |
Synonyms | MSA,TDH2A,TPX |
Molecular Weight | The secreted recombinant human TPO comprises 842 amino acids and has a predicted molecular mass of 93.8 kDa. The apparent molecular mass of rh TPO (1-846) is approximately 90-100 kDa in SDS-PAGE under reducing conditions. |
predicted N | Cys 15 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the human TPO (P07202-1) extracellular domain (Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was fused with a polyhistidine tag at the C-terminus. |
Bio-activity | |
Research Area | Developmental Biology |Metabolism |Pathways and Processes |Redox metabolism |Antioxidants |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3. |
Reference |