Human Tie2 / CD202b / TEK Protein (His & GST Tag)
CD202B,TIE-2,TIE2,VMCM,VMCM1
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| Catalog Number | P10700-H20B1 |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | CD202B,TIE-2,TIE2,VMCM,VMCM1 |
| Molecular Weight | The recombinant human TEK /GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human TEK (NP_000450) (Gln771-Ala1124) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Bio-activity | 1. No Kinase Activity 2. Measured by its binding ability in a functional ELISA. Immobilized human TEK (aa 771-1124) (P 10700-H20B1) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc (P 10691-H02H) with a linear range of 0.31-20 μg/ml. |
| Research Area | Immunology |Inflammation / Inflammatory Mediator |Inflammatory Cytokines & Chemoki and Receptors |Angiopoietins and Angiopoietin-like Protein Families |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature. |
| Reference |