Human UBASH3A Protein (aa 354-623, His Tag)

CLIP4,STS-2,TULA,TULA-1

• 100ug (NPP2567) Please inquiry

Catalog Number	P13847-H07E
Organism Species	Human
Host	E. coli
Synonyms	CLIP4,STS-2,TULA,TULA-1
Molecular Weight	The recombinant human UBASH3A consists of 286 amino acids and predicts a molecular mass of 32.2 KDa. It migrates as an approximately 30 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human UBASH3A (AAH69511.1) (Ala354-Asn623) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Signaling Pathway |G Protein Signaling |G Protein-Coupled Receptors (GPCRs) |Neurotransmitter G Protein-Coupled Receptors |
Formulation	Lyophilized from sterile PBS, pH7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	UBASH3A is a member of the T-cell ubiquitin ligand (TULA) family. This family consists of two members. Both of them can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of UBASH3A gene results in multiple transcript variants. It interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. UBASH3A promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. UBASH3A also exhibits negligigle protein tyrosine phosphatase activity at neutral pH. It may act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. It may also inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
Reference	Collingwood TS, et al. (2007) T-cell ubiquitin ligand affects cell death through a functional interaction with apoptosis-inducing factor, a key factor of caspase-independent apoptosis.". J. Biol. Chem. 282 (42): 30920-8. Smirnova EV, et al. (2008) TULA proteins bind to ABCE-1, a host factor of HIV-1 assembly, and inhibit HIV-1 biogenesis in a UBA-dependent fashion. Virology. 372(1):10-23. Wattenhofer M, et al. (2001) Isolation and characterization of the UBASH3A gene on 21q22.3 encoding a potential nuclear protein with a novel combination of domains. Hum Genet. 108(2):140-7.