Human UBE2A / HHR6A Protein (His Tag)
HHR6A,MRXS30,MRXSN,RAD6A,UBC2
- 100ug (NPP2569) Please inquiry
| Catalog Number | P12726-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | HHR6A,MRXS30,MRXSN,RAD6A,UBC2 |
| Molecular Weight | The recombinant human UBE2A consisting of 167 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an approximately 18.5 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met 1 |
| SDS-PAGE |  |
| Purity | > 80 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |Histone Modifying Enzymes |Ubiquitylation |E2 Ubiquitin Conjugating Enzymes |
| Formulation | Lyophilized from sterile PBS, 20% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene, a member of the family of ubiquitin-conjugating enzymes. In vivo, HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition, with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity, while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family. |
| Reference |