Human UBE2D1 / UBCH5 Protein (His Tag)

E2(17)KB1,SFT,UBC4/5,UBCH5,UBCH5A

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Catalog Number	P11432-H07E
Organism Species	Human
Host	E. coli
Synonyms	E2(17)KB1,SFT,UBC4/5,UBCH5,UBCH5A
Molecular Weight	The recombinant human UBE2D1 consisting of 161 amino acids and has a calculated molecular mass of 18.3 kDa. It migrates as an approximately16 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human UBE2D1 (P51668) (Ala 2-Met 147) was expressed, with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Immunology |Signal Transduction |Other Related Intracellular Topics |Checkpoint
Formulation	Lyophilized from sterile 25mM Tris, 100mM NaCl, 20% glycerol, 0.05% Tween 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74. Tokumoto M, et al. (2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200. Ohbayashi N, et al. (2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12. Gehrke SG, et al. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.