Human UBE2G1 Protein
E217K,UBC7,UBE2G
- 100ug (NPP4363) Please inquiry
| Catalog Number | P13200-HNCE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | E217K,UBC7,UBE2G |
| Molecular Weight | The recombinant human UBE2G1 consists of 172 amino acids and has a calculated molecular mass of 19.5 kDa as estimated in SDS-PAGE under reducing conditions. |
| predicted N | Gly |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human UBE2G1 (P62253) (Met 1-Glu 170) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |Histone Modifying Enzymes |Ubiquitylation |E2 Ubiquitin Conjugating Enzymes |
| Formulation | Lyophilized from sterile PBS, 10% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. |
| Reference |