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Human XRCC5 & XRCC6 Heterodimer Protein

XRCC5, XRCC6

Catalog Number PCT018-H07B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms XRCC5, XRCC6
Molecular Weight The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions.
predicted N His & His
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Bio-activity
Research Area
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background X-ray repair cross-complementing protein 5, also known as 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Lupus Ku autoantigen protein p86, TLAA and XRCC5, is a nucleus and chromosome which belongs to the ku80 family. XRCC5 is a single stranded DNA-dependent ATP-dependent helicase. XRCC5 has a role in chromosome translocation. 
Reference
  • Featherstone C. et al., 1999, Mutat Res. 434: 3-15.
  • Zhang Z. et al., 2001, J Biol Chem. 276: 38231-6.
  • Hsu H-L. et al.,  2002, DNA Repai.r 1: 225-35.
  • Roberts SA. et al., 2010, Nature. 464:1214-7.
  • Burkard TR. et al., 2011, BMC Syst Biol. 5: 17.