Human XRCC5 & XRCC6 Heterodimer Protein
XRCC5, XRCC6
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Catalog Number | PCT018-H07B |
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Organism Species | Human |
Host | Baculovirus-Insect Cells |
Synonyms | XRCC5, XRCC6 |
Molecular Weight | The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions. |
predicted N | His & His |
SDS-PAGE | |
Purity | > 90 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
Bio-activity | |
Research Area | |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | X-ray repair cross-complementing protein 5, also known as 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Lupus Ku autoantigen protein p86, TLAA and XRCC5, is a nucleus and chromosome which belongs to the ku80 family. XRCC5 is a single stranded DNA-dependent ATP-dependent helicase. XRCC5 has a role in chromosome translocation. |
Reference |