Human bFGF / FGF2 Protein
BFGF,FGF-2,FGFB,HBGF-2
- 100ug (NPP1081) Please inquiry
| Catalog Number | P10014-HNAE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | BFGF,FGF-2,FGFB,HBGF-2 |
| Molecular Weight | The recombinant human bFGF is a disulfide-linked homodimeric protein. The reduced monomer consists of 147 amino acids and has a calculated molecular mass of 16.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of bFGF is approximately 17 kDa. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus. |
| Bio-activity | Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.1 ng/ml. |
| Research Area | Immunology |Signal Transduction |Growth Factor & Receptor |Fibroblast Growth Factor (FGF) & Receptor |Fibroblast Growth Factor (FGF) |
| Formulation | Lyophilized from sterile 20mM Tris, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies. |
| Reference |