Human c-KIT / CD117 Protein (His Tag)
C-Kit,CD117,PBT,SCFR
- 100ug (NPP3616) Please inquiry
| Catalog Number | P11996-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | C-Kit,CD117,PBT,SCFR |
| Molecular Weight | The recombinant human KIT consists of 502 amino acids and predictes a molecular mass of 56.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKIT is approximately 75-85 kDa due to glycosylation. |
| predicted N | Gln 26 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human KIT isoform 2 (P10721-2) extracellular domain (Met 1-Thr 516) was expressed, with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human KIT at 20 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear ranger 1.28-6.4 ng/ml. |
| Research Area | Cancer |Signal transduction |Protein Kinase |Receptor Tyrosine Kinase (RTK) |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. |
| Reference |