Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein
gB
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| Catalog Number | P10202-VCCH1 |
|---|---|
| Organism Species | CMV |
| Host | Human Cells |
| Synonyms | gB |
| Molecular Weight | The recombinant glycoprotein B of human CMV consists of 813 amino acids and has a predicted molecular mass of 92 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB is approximately 130-140 kDa due to glycosylation. |
| predicted N | Ser 25 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was expressed, fused with five additional amino acids (DDDDK) at the C-terminus. |
| Bio-activity | |
| Research Area | Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Immune system illness | |
| Formulation | Lyophilized from sterile Tris 50mM, NaCl 100mM, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 907-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium. |
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