Influenza A H13N6 (A/black-headed gull/Sweden/1/1999) Hemagglutinin / HA Protein (His Tag)

Hemagglutinin, HA1

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Catalog Number	P40190-V08B
Organism Species	H13N6
Host	Baculovirus-Insect Cells
Synonyms	Hemagglutinin, HA1
Molecular Weight	The recombinant HA1 subunit of Influenza A virus (A/black-headed gull/Sweden/1/1999(H13N6)) consists 335 amino acids and predicts a molecular mass of 37.3 kDa.
predicted N	Asp 19
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE.
Protein Construction	A DNA sequence encoding the Influenza A virus (A/black-headed gull/Sweden/1/1999(H13N6)) hemagglutinin (AAV91213.1) (Met1-Arg342), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.5μg/mL for 1%GRBC.
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37