Call Now

Influenza A H1N1 (A/California/04/2009) Neuraminidase / NA (Active) (His Tag)

NA

Catalog Number P11058-V07B
Organism Species H1N1
Host Baculovirus-Insect Cells
Synonyms NA
Molecular Weight The recombinant influenza H1N1 virus neuraminidase (A/California/04/2009 (H1N1)) comprises 450 amino acids with the predicted molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions.
predicted N His
SDS-PAGE
Purity > 88 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mature form of influenza A virus (A/California/04/2009 (H1N1)) neuraminidase (ACP41107.1) (His 36-Lys 469) was fused with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.
Reference
  • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
  • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
  • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.