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Influenza A H1N1 (A/NewJersey/8/1976) Hemagglutinin / HA Protein (His Tag)

Hemagglutinin, HA

Catalog Number P40392-V08B
Organism Species H1N1
Host Baculovirus-Insect Cells
Synonyms Hemagglutinin, HA
Molecular Weight The recombinant HA1 subunit of the Influenza A virus (A/NewJersey/8/1976(H1N1)) consists 523 amino acids and predicts a molecular mass of 58.9 kDa.
predicted N Asp 18
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the Influenza A virus (A/NewJersey/8/1976(H1N1)) hemagglutinin (ACU80014.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity Measured by its ability to agglutinate guinea pig red blood cells.
HA titer is 0.5-2.5μg/mL for 1%GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37