Influenza A H1N1 (A/USSR/90/77) Hemagglutinin / HA Protein (His Tag)
HA
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| Catalog Number | P40134-V08B |
|---|---|
| Organism Species | H1N1 |
| Host | Baculovirus-Insect cells |
| Synonyms | HA |
| Molecular Weight | The recombinant hemagglutinin of Influenza A virus (A/USSR/90/1977(H1N1))comprises 523 amino acids and has a predicted molecular mass of 59.4 kDa. The apparent molecular mass of the protein is approximately 65.3 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Asp 18 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the Influenza A virus (A/USSR/90/77(H1N1)) hemagglutinin (P03453.2) (Met1-Gln529) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.01-0.04 μg/mL for 1% GRBC. |
| Research Area | Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness | |
| Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.5. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |