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Influenza A H3N8 (A/equine/Gansu/7/2008) Hemagglutinin Protein (HA1 Subunit) (His Tag)

Hemagglutinin, HA1

Catalog Number P40155-V08B
Organism Species H3N8
Host Baculovirus-Insect Cells
Synonyms Hemagglutinin, HA1
Molecular Weight The recombinant HA subunit of Influenza A virus (A/equine/Gansu/7/2008(H3N8)) consists 339 amino acids and predicts a molecular mass of 37.9 kDa.
predicted N Gln 17
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the Influenza A virus (A/equine/Gansu/7/2008(H3N8)) hemagglutinin (ACE81938.1) (Met1-Arg344), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System.
3. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.04-0.2 μg/mL for 1% GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference