Influenza A H5N1 (A/Anhui/1/2005) Hemagglutinin / HA Protein (His Tag)

HA

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Catalog Number	P11048-V08B
Organism Species	H5N1
Host	Baculovirus-Insect Cells
Synonyms	HA
Molecular Weight	The secreted recombinant hemagglutinin of Influenza A virus (A/Anhui/1/ 2005(H5N1) comprises 557 amino acids with the predicted molecular mass of 63 kDa. As a result of glycosylation, it migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.This recombinant HA proteins are polymers based on HPLC analysis (data not shown).
predicted N	Asp 17
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of Influenza A virus (A/Anhui/1/2005(H5N1) (ABD28180.1) hemagglutinin (Met 1-Gln 527,Ala 150Val) with cleavage site mutated (RERRRKR→TETR, HA1+HA2, uncleaved) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile 20mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37