Call Now

Influenza A H5N1 (A/Common magpie/Hong Kong/2256/2006) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P11700-V08H
Organism Species H5N1
Host Human Cells
Synonyms HA
Molecular Weight The secreted recombinant influenza A H5N1 (A/Common magpie/Hong Kong/2256/2006 (H5N1)) HA comprises 524 amino acids and has a predicted molecular mass of 59.6 kDa. As a result of glycosylation, it migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
predicted N Asp 17
SDS-PAGE
Purity > 97 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain (Met 1-Glu 530) of the influenza hemagglutinin (A/Common magpie/Hong Kong/2256/2006 (H5N1)) (ABJ96777.1) (HA1+HA2, uncleaved,with the cleavage site mutated (RRRKR-TETR)) was expressed, fused with a C-terminal polyhistidine tag.
Bio-activity
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37