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Influenza A H5N1 (A/Hubei/1/2010) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P40015-V08H
Organism Species H5N1
Host Human Cells
Synonyms HA
Molecular Weight The secreted recombinant hemagglutinin of influenza A virus H5N1 (A/Hubei/2011) comprises 525 amino acids and has a predicted molecular mass of 60.1 kDa. As a result of glycosylation, it migrates as an approximately 65-70 kDa band in non-reduced SDS-PAGE, and three bands (25, 45, 70 kDa) in reduced SDS-PAGE.
predicted N Asp 17
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of influenza A virus hemagglutinin (A/Hubei/1/2010 (H5N1)) (Met 1-Gln 530) (HA1 + HA2, cleaved) was fused with a polyhistidine tag at the C-terminus.
Bio-activity Measured by its ability to agglutinate human red blood cells.
HA titer is 0.5-4 μg/mL for 1% HRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference