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Influenza A H5N1 (A/bar-headed goose/Qinghai/1A/2005) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P40117-V08B
Organism Species H5N1
Host Baculovirus-Insect Cells
Synonyms HA
Molecular Weight The recombinant HA subunit of iInfluenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1) consists 522 amino acids and predicts a molecular mass of 59.6 kDa.
predicted N Asp 17
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the Influenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1)) hemagglutinin (ABF93441.1) (Met1-Gln527), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference