Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number	P40160-V08B
Organism Species	H5N1
Host	Baculovirus-Insect cells
Synonyms	HA
Molecular Weight	The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 526 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions.
predicted N	Asp 17
SDS-PAGE
Purity	> 60 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) was expressed with a C-terminal polyhistidine tag.
Bio-activity	1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 4-16 ng/mL for 1% GRBC.
Research Area	Microbiology \|Pathogenic microorganism \|viruses \|animal virus \|viral illness \|Viral tract respiratory illness \|
Formulation	Lyophilized from sterile 20 mM, Tris 500 mM NaCl, 10 % glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference