Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein (HA1+HA2) (His Tag)
Hemagglutinin, HA1+HA2
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| Catalog Number | P40160-V08B1 |
|---|---|
| Organism Species | H5N1 |
| Host | Baculovirus-Insect cells |
| Synonyms | Hemagglutinin, HA1+HA2 |
| Molecular Weight | The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524 amino acids and has a predicted molecular mass of 59.8 kDa. The apparent molecular mass of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Asp 17 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) with cleavage site mutated (RRRRK →TET, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC. |
| Research Area | Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness | |
| Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
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