Influenza A H5N1 (A/chicken/India/NIV33487/06) Hemagglutinin / HA Protein (His Tag)

HA

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Catalog Number	P11712-V08H
Organism Species	H5N1
Host	Human Cells
Synonyms	HA
Molecular Weight	The secreted recombinant influenza A H5N1 HA (A/chicken/India/NIV33487/2006 (H5N1)) comprises 524 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
predicted N	Asp 17
SDS-PAGE
Purity	> 94 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Gln 531) of the influenza A hemagglutinin (A/chicken/India/NIV33487/06 (H5N1)) (ABQ45850.1) (HA1+HA2, uncleaved, cleavage site mutated (RRRKKR-TETR)) was expressed, fused with a C-terminal polyhistidine tag.
Bio-activity	Measured by its ability to agglutinate human red blood cells. HA titer is 10-50 μg/mL for 1% HRBC.
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37