Call Now

Influenza A H5N1 (A/chicken/VietNam/NCVD-016/2008) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P40158-V08B
Organism Species H5N1
Host Baculovirus-Insect cells
Synonyms HA
Molecular Weight The recombinant hemagglutinin of Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1)) comprises 527 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 66.2 kDa in SDS-PAGE under reducing conditions.
predicted N Asp 17
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1))hemagglutinin(ACO07033.1) (Met1-Gln532) was expressed with a C-terminal polyhistidine tag.
Bio-activity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-4 ng/mL for 1% GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference