Influenza A H5N1 (A/chicken/VietNam/NCVD-016/2008) Hemagglutinin / HA Protein (His Tag)
HA
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Catalog Number | P40158-V08B |
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Organism Species | H5N1 |
Host | Baculovirus-Insect cells |
Synonyms | HA |
Molecular Weight | The recombinant hemagglutinin of Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1)) comprises 527 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 66.2 kDa in SDS-PAGE under reducing conditions. |
predicted N | Asp 17 |
SDS-PAGE | |
Purity | > 85 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the Influenza A virus (A/chicken/VietNam/NCVD-016/2008(H5N1))hemagglutinin(ACO07033.1) (Met1-Gln532) was expressed with a C-terminal polyhistidine tag. |
Bio-activity | 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-4 ng/mL for 1% GRBC. |
Research Area | Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness | |
Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | |
Reference |