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Influenza A H6N2 (A/duck/Shantou/83/2000) Hemagglutinin / HA Protein (His Tag)

Hemagglutinin, HA1

Catalog Number P40166-V08B
Organism Species H6N2
Host Baculovirus-Insect Cells
Synonyms Hemagglutinin, HA1
Molecular Weight The recombinant Influenza A virus (A/duck/Shantou/83/2000(H6N2)) hemagglutinin consists 524 amino acids and predicts a molecular mass of 59.5 kDa.
predicted N Asp 17
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the Influenza A virus (A/duck/Shantou/83/2000(H6N2)) hemagglutinin (ADG44842.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37