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Influenza A H7N7 (A/Netherlands/219/03) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P11082-V08B
Organism Species H7N7
Host Baculovirus-Insect Cells
Synonyms HA
Molecular Weight The secreted recombinant hemagglutinin of influenza A virus (A/Netherlands/219/03 (H7N7)) comprises 530 amino acids and has a predicted molecular mass of 59 kDa as estimated in SDS-PAGE under reducing conditions.
predicted N Asp 26
SDS-PAGE
Purity > 87 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of Influenza A virus (A/Netherlands/219/03 (H7N7)) (AAR02640.1) hemagglutinin (Met 8-Phe 544) (Native, HA1+HA2) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its ability to agglutinate guinea pig red blood cells.
HA titer is 0.05-0.3μg/ml for 1% GRBC
2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference