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Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P40126-V08B
Organism Species H7N9
Host Baculovirus-Insect cells
Synonyms HA
Molecular Weight The recombinant hemagglutinin of Influenza A virus (A/Shanghai/4664T/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.
predicted N Asp 19
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the Influenza A virus (A/Shanghai/4664T/2013(H7N9)) hemagglutinin (AGI60292.1) (Met1-Val524) was expressed with a C-terminal polyhistidine tag.
Bio-activity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference