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Influenza A H7N9 (A/Zhejiang/DTID-ZJU10/2013) Hemagglutinin / HA Protein (His Tag)

HA

Catalog Number P40325-V08B
Organism Species H7N9
Host Baculovirus-Insect Cells
Synonyms HA
Molecular Weight The recombinant HA subunit of influenza A virus (A/Zhejiang/DTID-ZJU10/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa.
predicted N Asp 19
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the influenza A virus (A/Zhejiang/DTID-ZJU10/2013(H7N9)) hemagglutinin (AHA11500.1) (Met1-Val524), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System.
3. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.2-1 μg/mL for 1% GRBC.
Research Area Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol,pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference