Influenza A H9N2 (A/Hong Kong/35820/2009) Hemagglutinin / HA Protein (His Tag)
HA
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Catalog Number | P40174-V08B |
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Organism Species | H9N2 |
Host | Baculovirus-Insect cells |
Synonyms | HA |
Molecular Weight | The recombinant hemagglutinin of Influenza A virus (A/Hong Kong/35820/ 2009(H9N2)) comprises 516 amino acids and has a predicted molecular mass of 58.5 kDa. The apparent molecular mass of the protein is approximately 59.6 kDa in SDS-PAGE under reducing conditions. |
predicted N | Asp 19 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the Influenza A virus (A/Hong Kong/35820/ 2009(H9N2)) hemagglutinin (ADC41853.1) (Met1-Lys523) was expressed with a C-terminal polyhistidine tag. |
Bio-activity | 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.2-0.8 ng/mL for 1% GRBC. |
Research Area | Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness | |
Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.5. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | |
Reference |