Marmoset IL-23 (IL23A & IL12B Heterodimer) Protein
IL23A & IL12B
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| Catalog Number | PCT037-C08H |
|---|---|
| Organism Species | Marmoset |
| Host | Human Cells |
| Synonyms | IL23A & IL12B |
| Molecular Weight | The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE. |
| predicted N | Arg 20 & Ile 23 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized marmoset IL23A-His+marmoset IL12B-His (PCT037-C08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (P11674-H08H). The EC50 of biotinylated human IL12RB1-His (P11674-H08H) is 55-129 μg/ml. |
| Research Area | Cancer |Invasion microenvironment |Adhesion molecule |Extracelluar matrix |others in ECM |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
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