Mouse ACO2 / Aconitase 2 Protein (His & GST Tag)

Aco-2,Aco3,D10Wsu183e

Catalog Number	P51015-M20B
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	Aco-2,Aco3,D10Wsu183e
Molecular Weight	The secreted recombinant mouse ACO2/GST chimera consists of 990 amino acids and has a calculated molecular mass of 110 kDa. The recombinant protein migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse ACO2 (Q99KI0) (Gln 28-Gln 780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area	Immunology \|Signal Transduction \|Metabolism \|Types of disease \|Metabolism in Cancer
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% gly, 0.5mM GSH, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Robbins AH, et al. (1989) The structure of aconitase. Proteins. 5 (4): 289-312. Lauble H, et al. (1992) Crystal structures of aconitase with isocitrate and nitroisocitrate bound. Biochemistry. 31 (10): 2735-48. Robbins AH, et al. (1989) Structure of activated aconitase: formation of the 4Fe-4S cluster in the crystal. Proc Natl Acad Sci. 86 (10): 3639-43.