Mouse ACVR2A / ActrIIa Protein (His & Fc Tag)

ActrIIa,Acvr2,TactrII

100ug (NPP1040) Please inquiry

Catalog Number	P50613-M03H
Organism Species	Mouse
Host	Human Cells
Synonyms	ActrIIa,Acvr2,TactrII
Molecular Weight	The secreted recombinant mouse ACVR2A/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 363 amino acids and has a calculated molecular mass of 41.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-65 kDa band in SDS-PAGE under reducing conditions.
predicted N	Ala 20
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of mouse ACVR2A (NP_031422.3) (Met 1-Pro 134) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 20-60 ng/mL in the presence of 10 ng/mL recombinant Activin A.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Growth Factor & Receptor \|Transforming Growth Factor Beta (TGF-beta) Superfamily \|TGF-beta Superfamily Receptors \|
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.
Reference	Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18. Chung H, et al. (2008) Mutation rates of TGFBR2 and ACVR2 coding microsatellites in human cells with defective DNA mismatch repair. PloS one 3(10): e3463. Fitzpatrick E, et al. (2009) Genetic association of the activin A receptor gene (ACVR2A) and pre-eclampsia. Molecular human reproduction 15(3):195-204. Roten LT, et al. (2009) Association between the candidate susceptibility gene ACVR2A on chromosome 2q22 and pre-eclampsia in a large Norwegian population-based study (the HUNT study). EJHG. 17(2): 250-7.