Mouse ACVR2B Protein (His & Fc Tag)

4930516B21Rik,ActRIIB

100ug (NPP1045) Please inquiry

Catalog Number	P50173-M03H
Organism Species	Mouse
Host	Human Cells
Synonyms	4930516B21Rik,ActRIIB
Molecular Weight	The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.
predicted N	Ser l9
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Thr 134) of mouse ACVR2B (NP_031423.1) precursor was fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity	1. Measured by its ability to bind biotinylated Human INHBA-his (P10429-H08H) in functional ELISA. 2. Measured by its ability to bind biotinylated mouse INHBA-his (P50659-M08H) in functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.
Research Area	Cancer \|Signal transduction \|Cytoskeleton / ECM \|Cytoskeletal Proteins \|Microfilaments \|Actin etc \|
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
Reference	Kosaki R, et al. (1999) Left-right axis malformations associated with mutations in ACVR2B, the gene for human activin receptor type IIB. Am J Med Genet. 82(1):70-6. Dupont S, et al. (2001) No evidence for linkage or for diabetes-associated mutations in the activin type 2B receptor gene (ACVR2B) in French patients with mature-onset diabetes of the young or type 2 diabetes. Diabetes 50(5):1219-21. Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18. Walsh S, et al. (2007) Activin-type II receptor B (ACVR2B) and follistatin haplotype associations with muscle mass and strength in humans. J Appl Physiol. 102(6):2142-8.