Mouse ALK-3 / BMPR1A Protein (His Tag)

1110037I22Rik,ALK3,AU045487,Bmpr,BMPR-IA

100ug (NPP3175) Please inquiry

Catalog Number	P50078-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	1110037I22Rik,ALK3,AU045487,Bmpr,BMPR-IA
Molecular Weight	The recombinant mouse ALK3 consists of 140 amino acids after removal of the signal peptide and has a predicted molecular mass of 15.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ALK3 is approximately 30 kDa due to glycosylation.
predicted N	Gln 24
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of mouse ALK3 (NP_033888.2) precursor was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED₅₀ for this effect is typically 0.5-2 μg/ml in the presence of 50 ng/ml of recombinant human BMP4.
Research Area	Cardiovascular \|Angiogenesis \|Cytokine & Receptor \|Transforming Growth Factor Beta (TGF-beta) Superfamily \|TGF-beta Superfamily Receptors
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-β) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.
Reference	Gaussin V, et al. (2002) Endocardial cushion and myocardial defects after cardiac myocyte-specific conditional deletion of the bone morphogenetic protein receptor ALK3. Proc Natl Acad Sci U S A. 99(5): 2878-83. Eblaghie MC, et al. (2006) Evidence that autocrine signaling through Bmpr1a regulates the proliferation, survival and morphogenetic behavior of distal lung epithelial cells. Dev Biol. 291(1): 67-82. Sun J, et al. (2007) Deficient Alk3-mediated BMP signaling causes prenatal omphalocele-like defect. Biochem Biophys Res Commun. 360(1): 238-43. Lee NY, et al. (2009) The transforming growth factor-beta type III receptor mediates distinct subcellular trafficking and downstream signaling of activin-like kinase (ALK)3 and ALK6 receptors. Mol Biol Cell. 20(20): 4362-70.