Mouse Anti-Human CD11a

100ug (NP20116) Please inquiry

Antibody type:Flowcytometry

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:FCM,IF

Purify method:

Species:Human

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Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5H3-F6

Dilution:

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Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01M PBS, pH 7.4, 0.09%(w/v) NaN3

Background:1. 取100μl EDTA抗凝的人外周血加入流式测定管底部;2. 加入适量纯化抗体与全血混匀，室温孵育30分钟;3. 加入2 ml 1×红细胞裂解液混匀后避光置10分钟,溶解红细胞;4. 将试管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;6. 加入适量荧光标记抗小鼠免疫球蛋白试剂，室温避光孵育20分钟;7. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:5H3-F6单克隆抗体识别180 kDa的CD11a分子，即白细胞功能相关抗原-1α 链(LFA-1α)，存在于所有白细胞包括T细胞、B细胞、单核细胞和粒细胞上，但不存在于非造血组织和血小板上。CD11/CD18 (LFA-1) 是整合素超家族的一个成员，是白细胞黏附受体，在细胞-细胞接触如淋巴细胞黏附、NK和 T细胞的溶细胞作用和T细胞增殖中是必须的。CD11/CD18也参与白细胞和内皮细胞之间的相互作用。此克隆与非人灵长类动物有交叉反应。CD11a抗体已用于降低免疫缺陷儿童骨髓移植后移植物衰竭的发生率。

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.