Mouse Anti-Human CD11a (PE)
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Antibody type:Flowcytometry
Label:PE
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM,IF
Purify method:
Species:Human
Gene Name:
Synonyms:
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:6B6-E1
Dilution:
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:2-8℃避光保存，勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定 Mincho
Molar Function:识别CD11b/18双面体中的α链,分子量为165kD, 是补体C3bi受体. CD11b抗原表达于激活淋巴细胞,单核细胞,粒细胞与NK细胞亚类上.它与细胞-细胞间以及细胞-介质间相互作用有关。这个抗体能明显抑制fMLP反应中的粒细胞凝集。该抗体可用于福尔马林固定,石蜡包埋切片的免疫组化染色。用途：研究补体功能;鉴定白细胞粘附缺陷症-1(LAD-1)。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.