Mouse Anti-Human CD13 (PE)
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Antibody type:Flowcytometry
Label:PE
Modification:Unmodification
Isotype:IgG2a
Host:Mouse
Application:FCM,IF
Purify method:
Species:Human
Gene Name:
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Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:7C4-D5
Dilution:
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Location:
Concentration:
Sequence Similarity:
Gene Id:
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Unigene:
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Tissue specificity:
Storage:2-8℃避光保存,勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100μl EDTA抗凝的人外周血加入流式测定管底部;2. 加入2 ml 1×红细胞裂解液混匀后避光置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);3. 将试管置离心机中以1000 rpm离心5分钟后弃上清;4. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;5. 加入1ml 固定液,吹打均匀,固定40min;6. 加入1ml 固定破膜剂,吹打均匀,1000 rpm离心5分钟,弃上清;7. 加入8ml 固定破膜剂,吹打均匀,室㿿
Molar Function:识别150 kDa的CD13分子,即氨基肽酶N(APN)。CD13抗原表达于早期定向祖细胞、成熟粒细胞、单核细胞、内皮细胞、上皮细胞、骨髓基质细胞、破骨细胞和小部分LGL淋巴细胞,但淋巴细胞、血小板和红细胞不表达。CD13是RNA病毒特异株的受体,并在人类巨细胞病毒(CMV)和其靶细胞间的相互作用中起重要作用。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.