Mouse Anti-Human CD14 (Biotin)
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Antibody type:Flowcytometry
Label:Biotin
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM,IF
Purify method:
Species:Human
Gene Name:
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Gene Infomation:
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Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:2H4-H10
Dilution:
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Tissue specificity:
Storage:2-8℃避光保存，勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入SAV-PE或SAV-FITC，室温避光孵育20分钟；7. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]
Molar Function:2H4-H10单克隆抗体识别CD14抗原，一个53-55 kDa的分子，是人类脂多糖（LPS-内毒素）和血清LPS-结合蛋白(LPB)复合物的高亲和力受体。CD14抗原在单核/巨噬细胞表面有强表达，在粒细胞上有弱表达，但不存在于髓系祖细胞上。CD14是内毒素的受体；当单核细胞激活时释放细胞因子如TNF和上调细胞表面分子包括黏附分子。这一克隆与非人灵长类动物有交差反应。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.