Mouse Anti-Human CD22 (FITC)
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Antibody type:Flowcytometry
Label:FITC
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM,IF
Purify method:
Species:Human
Gene Name:
Synonyms:
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:5H9-B8
Dilution:
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:2-8℃避光保存,勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞,1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]
Molar Function:识别一个140 kDa 的特异性B淋巴细胞抗原CD22。CD22抗原存在于不同阶段发育的B淋巴细胞胞浆内,在成熟B淋巴细胞,仅表达于细胞表面。CD22抗原也表达在大多数B细胞白血病和淋巴瘤;但在任何T细胞白血病不表达。CD22分子参与B细胞激活作用。与其它B淋巴细胞抗原如CD10,CD19和CD20不同,CD22抗原还存在于浆细胞样淋巴细胞上,但在完全成熟的浆细胞上减少。CD22是黏附分子,在B细胞活化过程中作为信号分子而发挥重要作用。CD22抗体可用于治疗白血病和淋巴瘤。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.