Mouse Anti-Human CD25 (Biotin)

100ug (NP20184) Please inquiry

Antibody type:Flowcytometry

Label:Biotin

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:FCM,IF

Purify method:

Species:Human

Gene Name:

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Antigen Synonyms:

Clonality:Monclonal antibody

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Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7C3-G6

Dilution:

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Sequence Similarity:

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Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入SAV-PE或SAV-FITC，室温避光孵育20分钟； 7. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清; 8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:7C3-G6识别CD25抗原，即低亲和性IL-2受体（IL-2R）的α链。CD25抗原表达于激活的T、B、NK细胞和单核细胞，也表达在部分胸腺细胞、HTLV-1转化的T细胞系、EBV 转化的 B细胞、髓系前体细胞和少突细胞上。高亲和性IL-2受体是由α( 55 kDa, CD25 )、β( 75 kDa ,CD122 )、γ( 70 kDa, CD132 )三个亚单位以非共价键结合而成。IL-2 与IL-2R相互作用诱导T、 B 、NK细胞和巨噬细胞的活化和增殖。CD4+/CD25+细胞可能直接调控反应性T细胞的功能。

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.