Mouse Anti-Human CD3 (Biotin)

Antibody type:Flowcytometry

Label:Biotin

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:FCM,FA,IHC,ICC,IF,WB,ELISA

Purify method:

Species:Human

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C12-H11

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入SAV-PE或SAV-FITC，室温避光孵育20分钟；7. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:识别一个140 kDa 的特异性B淋巴细胞抗原CD22。CD22抗原存在于不同阶段发育的B淋巴细胞胞浆内，在成熟B淋巴细胞，仅表达于细胞表面。CD22抗原也表达在大多数B细胞白血病和淋巴瘤；但在任何T细胞白血病不表达。CD22分子参与B细胞激活作用。与其它B淋巴细胞抗原如CD10，CD19和CD20不同，CD22抗原还存在于浆细胞样淋巴细胞上，但在完全成熟的浆细胞上减少。CD22是黏附分子，在B细胞活化过程中作为信号分子而发挥重要作用。CD22抗体可用于治疗白血病和淋巴瘤。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.