Mouse Anti-Human CD3 (PE)
- 100ug (NP19946) Please inquiry
Antibody type:Flowcytometry
Label:PE
Modification:Unmodification
Isotype:IgG2a
Host:Mouse
Application:FCM,FA,IHC,ICC,IF,WB,ELISA
Purify method:
Species:Human
Gene Name:
Synonyms:
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:6C12-H11
Dilution:
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:2-8℃避光保存，勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100μl EDTA抗凝的人外周血加入流式测定管底部;2. 加入2 ml 1×红细胞裂解液混匀后避光置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);3. 将试管置离心机中以1000 rpm离心5分钟后弃上清;4. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;5. 加入1ml 固定液，吹打均匀，固定40min；6. 加入1ml 固定破膜剂，吹打均匀，1000 rpm离心5分钟,弃上清;7. 加入2ml 固定破膜剂，吹打均匀，室Àდpდ
Molar Function:识别一个90 kDa的B细胞抗原，大约5-25%的人外周血淋巴细胞表达此抗原。CD19抗原存在与大多数成熟阶段的B淋巴细胞上，从最早的前B细胞中的Ig基因重排到成熟细胞及恶性B细胞，但发育到浆细胞阶段则消失。CD19抗体不与T淋巴细胞，粒细胞和单核细胞反应。CD19是一重要的调节B淋巴细胞发育、活化和分化的信号转导分子，也可作为白血病的分型标记。这一克隆与非人灵长类动物有交叉反应。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.