Mouse Anti-Human CD4 (PE)

Antibody type:Flowcytometry

Label:PE

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:FCM,IHC,IF,IP

Purify method:

Species:Human

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4E11-G7

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定

Molar Function:识别55 kDa的人类CD4分子，此抗原表达于80-95%的正常胸腺细胞、28-58%正常外周血T淋巴细胞（Th/i细胞）、85-90%外周血单核细胞，并且在组织巨噬细胞上有低水平表达。CD4分子是MHC II类分子限制性抗原介导T细胞激活的共受体，也是HIV逆转录病毒的第一受体。CD4在调控T-B淋巴细胞黏附和胸腺分化过程中起重要作用。此克隆与非人灵长类动物间有交叉反应。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.