Mouse Anti-Human CD40 (FITC)

Antibody type:Flowcytometry

Label:FITC

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:FCM,IF

Purify method:

Species:Human

Gene Name:

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Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7G2-C10

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

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Unigene:

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Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:7G2-C10识别CD40( Bp50 )分子，TNF受体家族的一个成员，分子量为48 kDa。CD40是全B细胞标记，表达于从前B细胞到浆细胞各个B细胞发育阶段、树突细胞和CD34+造血祖细胞，但单核细胞和粒细胞不表达。CD40分子参与B细胞的生长、分化；Ig的异型转换；是树突细胞的上调黏附分子；促进巨噬细胞和树突细胞分泌细胞因子。据报道，CD40抗体可与抗-μ或佛波醇酯共同刺激B细胞增殖；用于监测移植排斥及T细胞和Ab介导的自身免疫病。此克隆与非人灵长类动物有交叉反应。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.