Mouse Anti-Human CD40 (PE/Cy5)
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Antibody type:Flowcytometry
Label:PE/Cy5
Modification:Unmodification
Isotype:IgG2a
Host:Mouse
Application:FCM,IF
Purify method:
Species:Human
Gene Name:
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Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:7G2-C10
Dilution:
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Location:
Concentration:
Sequence Similarity:
Gene Id:
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Unigene:
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Tissue specificity:
Storage:2-8℃避光保存,勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞,1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]
Molar Function:5H3-F6单克隆抗体识别180 kDa的CD11a分子,即白细胞功能相关抗原-1α 链(LFA-1α),存在于所有白细胞包括T细胞、B细胞、单核细胞和粒细胞上,但不存在于非造血组织和血小板上。CD11/CD18 (LFA-1) 是整合素超家族的一个成员,是白细胞黏附受体,在细胞-细胞接触如淋巴细胞黏附、NK和 T细胞的溶细胞作用和T细胞增殖中是必须的。CD11/CD18也参与白细胞和内皮细胞之间的相互作用。此克隆与非人灵长类动物有交叉反应。CD11a抗体已用于降低免疫缺陷儿童骨髓移植后移植物衰竭的发生率。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.