Mouse Anti-Human CD41

100ug (NP20039) Please inquiry

Antibody type:Flowcytometry

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:FCM

Purify method:

Species:Human

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Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4H7-B6

Dilution:

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Storage:短期内经常使用时，请将抗体2-8℃保存，至少可以保存6个月。若长期保存，请将抗体分装后-20℃至-80℃保存，切忌反复冻融。

Buffer condition:0.01M PBS,0.09% NaN3,pH7.4

Background:1. 分离血小板:将全血样本以800 rpm离心15分钟，取上清以2000 rpm离心5分钟，弃上清;2. 用PBS洗液调节血小板浓度约1×106个/ml备用；3. 准确取100ul调节好浓度的血小板悬液到流式测定管底部；4. 加入适量纯化抗体(需滴定)与全血混匀，室温孵育30分钟;5. 向样本管中加入2ml PBS洗液，混匀后以1000rpm离心5分钟，弃上清；6、加入适量荧光标记抗小鼠免疫球蛋白试剂，室温避光孵育20分钟;6. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;7. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:4H7-B6单抗识别钙依赖性CD41/CD61（GPⅡb/Ⅲa）复合物，分子量为135/120 kDa，表达于正常血小板和巨核细胞上。CD41抗原是纤维蛋白原、vWf、纤维蛋白酶原和玻璃体结合蛋白的受体，并介导血小板黏附和聚集。4H7-B6单抗可完全抑制ADP、肾上腺素和胶原蛋白介导的血小板激活，并部分抑制软脂酸十六酯和凝血酶诱导的血小板激活，在血小板和巨核细胞的形态学和生理学研究中非常有用。CD41可用于Glanzmann's血小板机能不全和巨核细胞白血病的诊断,同种抗体缺失及抗血栓治疗。

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.