Mouse Anti-Human CD41
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Antibody type:Flowcytometry
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM
Purify method:
Species:Human
Gene Name:
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Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:4C12-B1
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Storage:短期内经常使用时,请将抗体2-8℃保存,至少可以保存6个月。若长期保存,请将抗体分装后-20℃至-80℃保存,切忌反复冻融。
Buffer condition:0.01M PBS,0.09% NaN3,pH7.4
Background:1. 分离血小板:将全血样本以800 rpm离心15分钟,取上清以2000 rpm离心5分钟,弃上清;2. 用PBS洗液(Cat.:FXP005)调节血小板浓度约1×106个/ml备用;3. 准确取100ul调节好浓度的血小板悬液到流式测定管底部;4. 加入适量纯化抗体(需滴定)与全血混匀,室温孵育30分钟;5. 向样本管中加入2ml PBS洗液,混匀后以1000rpm离心5分钟,弃上清;6、加入适量荧光标记抗小鼠免疫球蛋白试剂,室温避光孵育20分钟;6. 加入2 ml PBS洗液(Cat.:FXP005)重悬细胞,1000 rpm离心5分钟,弃上清;7. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3;Cat.:FXP005][细胞固定液:2%甲醛溶液]
Molar Function:4C12-B1单抗识别钙依赖性CD41/CD61(GPⅡb/Ⅲa)复合物,分子量为135/120 kDa,表达于正常血小板和巨核细胞上。CD41抗原是纤维蛋白原、vWf、纤维蛋白酶原和玻璃体结合蛋白的受体,并介导血小板黏附和聚集。HIP8单抗可完全抑制ADP、肾上腺素和胶原蛋白介导的血小板激活,并部分抑制软脂酸十六酯和凝血酶诱导的血小板激活,在血小板和巨核细胞的形态学和生理学研究中非常有用。CD41可用于Glanzmann's血小板机能不全和巨核细胞白血病的诊断,同种抗体缺失及抗血栓治疗。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.