Mouse Anti-Human CD45 (FITC)
- 100ug (NP19959) Please inquiry
Antibody type:Flowcytometry
Label:FITC
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:IHC,Blocking,FCM,FunctionalStudies,ICC,IP,WB,CyTOF®,IF
Purify method:
Species:Human
Gene Name:
Synonyms:
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:3G6-C3
Dilution:
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:2-8℃避光保存,勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞,1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定x03
Molar Function:识别CD45分子,一个180-220 kDa的白细胞共同抗原(LCA)。CD45抗原高表达于所有造血细胞,包括T、B淋巴细胞、NK和树突细胞,但在非造血细胞中不表达。据报道CD45抗体与成熟外周血红细胞和血小板有弱反应。CD45分子是蛋白络氨酸磷酸酶受体,在T、B细胞抗原受体介导的激活过程中起重要作用。是免疫抑制抗体治疗的耙分子。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.