Mouse Anti-Human CD5 (PE)
- 100ug (NP19944) Please inquiry
Antibody type:Flowcytometry
Label:PE
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM,IF,WB,CyTOF®
Purify method:
Species:Human
Gene Name:
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Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:1C5-F11
Dilution:
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Location:
Concentration:
Sequence Similarity:
Gene Id:
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Unigene:
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Tissue specificity:
Storage:2-8℃避光保存,勿冰冻。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞,1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定 Mincho
Molar Function:识别58 kDa的T细胞抗原分子,CD5抗原是清道夫受体超家族的一个成员,在胸腺细胞和所有成熟T淋巴细胞上表达,也存在于一小部分在婴儿期增殖的成熟B淋巴细胞(B1a 细胞)和一些自身免疫性疾病和某些慢性B淋巴细胞白血病(B-CLL)细胞上。CD5分子为一双重受体,既是胸腺细胞和B1a细胞的抑制性信号受体,也是共刺激信号受体。CD5介导的细胞间反应可能影响胸腺细胞的成熟和选择。CD5也是一些B淋巴细胞增殖性疾病(B-CLL、外周区淋巴瘤、毛细胞白血病等)的表型标记。外周血CD3 +/ CD5-T细胞增加与GVHD的出现相一致。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.