Mouse Anti-Human CD54

Antibody type:Flowcytometry

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:FCM

Purify method:

Species:Human

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3B8-E9

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:短期内经常使用时，请将抗体2-8℃保存，至少可以保存6个月。若长期保存，请将抗体分装后-20℃至-80℃保存，切忌反复冻融。

Buffer condition:0.01M PBS,0.09% NaN3,pH7.4

Background:1. 取100μl EDTA抗凝的人外周血加入流式测定管底部;2. 加入适量纯化抗体与全血混匀，室温孵育30分钟;3. 加入2 ml 1×红细胞裂解液混匀后避光置10分钟,溶解红细;4. 将试管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;6. 加入适量荧光标记抗小鼠免疫球蛋白试剂，室温避光孵育20分钟;7. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:3B8-E9单抗识别CD54分子，即90 kDa 的细胞间黏附分子－1(ICAM-1)。CD54分子表达于激活的内皮细胞（强）、激活的T、B淋巴细胞和单核细胞（中等）及ATL和一些实体瘤细胞上。CD54介导上皮细胞、纤维母细胞和内皮细胞的作用，细胞因子如TNF,、IL-1和IFN-g可增强其作用；CD54还是鼻病毒或受疟疾感染的红细胞的受体。CD11a/CD18 或CD11bCD18 与CD54分子结合引起免疫反应和继发炎症。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.