Mouse Anti-Human CD55 (PE)

Antibody type:Flowcytometry

Label:PE

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IF,FCM,IHC

Purify method:

Species:Human

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7F10-E3

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞(推荐: RBC Lysing Solution[10×]);4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入010 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定 Mincho

Molar Function:识别CD55, 一个70 kDa的 GPI锚定的单链糖蛋白，即衰变加速因子(DAF)。CD55广泛分别于造血细胞（包括红细胞和NK细胞）和一些非造血细胞上。DAF通过防止补体成分的瀑布式反应扩大化来保护细胞免受自身补体的损伤。PIG-A 基因缺陷可导致GPI关联蛋白如CD55缺失和获得性溶贫，即阵发性睡眠性血红蛋白尿(PNH)。细胞表面保护性蛋白缺失导致PNH患者的红细胞对补体介导的溶血作用非常敏感。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.